Nanofabricated Arrays of Thermoelectric Sensors and Actuators:  The objective of this study is to nanofabricate arrays of probes that take advantage of thermoelectric principles to measure and modulate temperature changes on the cellular level. Our primary focus will be on solving the packaging and instrumentation issues with the thermoelectric sensors and actuators in order to obtain working instruments. In the final device arrays, probes for temperature measurement and temperature modulation will be interspersed and all probes will be individually addressable. The devices, if successful, will give the biologist or bioengineer highly localized control of the temperature measurement over large areas. In order to demonstrate the potential of the new instruments, we will use them to establish the physicochemical changes that occur during freezing of artificial tissues (ATs). Using the nanofabricated thermoelectric sensors and actuators, we will determine if there is a link between critical thermophysical events during freezing and the ultimate mechanical properties as well as viability of the ATs post-freeze. Our assumption is that freezing-induced cellular level thermal/biophysical events determine the ultimate post-thaw viability and the mechanical properties of the artificial tissue. The results from the project will help to further improve our understanding of the freezing process in tissue systems, where such data is currently scarce.

Publications & Presentations:

Journal Papers:

D. Pinisetty, C. Huang, Q. Dong, T.R. Tiersch and R. V. Devireddy. "Subzero Water Permeability Parameters and Optimal Freezing Rates for Sperm Cells of the Southern Platyfish, Xiphophorus Maculatus",  Cryobiology, Vol.50, pp:250-263

D. Pinisetty, D. Moldovan and R.V. Devireddy. "The Effect of Methanol on Lipid Bilayers – An Atomistic Investigation", Annals of Biomedical Engineering. 34: 1442-1451 (2006).

D. Moldovan, D. Pinisetty and R.V. Devireddy. "Entropy-Driven Pore Growth in Lipid Bilayer Membranes in the Presence of Edge-Active Agents",  Physical Review Letters, In Review.
 

Refereed Conference Papers:

 S. Mittal, D. Pinsetty and R.V. Devireddy. "Desiccation Tolerance of Human Adipose Tissue Derived Adult Stem Cells", 19th National and 8th ISHMT-ASME Heat and Mass Transfer Conference 2008, Hyderabad, India, CD-ROM Publication.

 D. Pinisetty, R. Alapati, D. Moldovan and R.V. Devireddy. "Molecular Dynamics Simulation Studies of Pore Formation in Lipid Bilayers in the Presence of Dimethylsulfoxide",  ASME IMECE 2007, Seattle, WA, CD-ROM Publication.

 D. Davis, D. Pinisetty, M. Moldovan, A. Prabhakar, D. Young, R.V. Devireddy, M. Murphy and E. Podlaha. "Electrodeposited CoNiFe/Cu and Bi2Te3 Nanotubes",  Presented at the 211th Meeting of the Electrochemical Society 2007, Chicago, Illinois.

 D. Pinisetty, J. Saenz, R. A. Godke and R.V. Devireddy. "Theoretical Predictions of Optimal Cooling Rates for Cryopreservation of Caprine Sperm".  ASME Summer Bioengineering Meeting 2006, Amelia Island, CD-ROM Publication.

D. Pinisetty, D. Moldovan and R.V. Devireddy. "An Atomistic Investigation of Dimethylsulfoxide Interacting with DPPC, DMPC and POPC Lipid Bilayers".  ASME Summer Bioengineering Meeting, Amelia Island 2006, CD-ROM Publication.

D. Pinisetty, D. Moldovan and R.V. Devireddy. "Effect of DMSO on POPC and DMPC Lipid Bilayers: An Atomistic Investigation".  Cryobiology, 51: 413-414.

 S. Mittal, D. Pinisetty and R.V. Devireddy. "Drying Storage of Adipose Derived Adult Stem Cells". Cryobiology, 2005,  51: 401-402.

D. Pinisetty, D. Moldovan and R. V. Devireddy. "Molecular Dynamic Simulations of Water Transport Through Lipid Bilayers In the Presence of Sugars". 38th National Heat Transfer Conference 2005, San Francisco, CD-ROM Publication.